Dynamic modification of proteins with ubiquitin is an important regulator of the cellular response to DNA damage. Several recent studies have pointed to important, but so far relatively unexplored roles of ubiquitin in the timely removal and/or degradation of DNA repair factors and chromatin-associated proteins during and/or after DNA repair.
We have developed a mass spectrometric (MS) method to monitor site-specific changes in ubiquitylation after cellular perturbations and have employed this method to perform a first systematic investigation of protein ubiquitylation following DNA damage induced by UV light irradiation. We are currently employing MS-based proteomics as well as biochemistry and cell biology to study the roles of protein ubiquitylation in the regulation of protein homeostasis in the nucleus after genotoxic stress.
Since 2013: Emmy Noether Group Leader, Institute of Molecular Biology (IMB), Mainz
2010 - 2013: Postdoctoral Researcher, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
2011: PhD in Biology, Goethe University Frankfurt
2007: Masters in Molecular Biology, University of Zagreb
Selected publications by Petra Beli
Bekbulat F*, Schmitt D*, Feldmann A, Huesmann H, Eimer S, Juretschke T, Beli P, Behl C# and Kern A# (2019) RAB18 Loss Interferes With Lipid Droplet Catabolism and Provokes Autophagy Network Adaptations. J Mol Biol, doi: 10.1016/j.jmb.2019.12.031 (*indicates joint contribution, #indicates joint correspondence) Link
Hildebrandt A, Brüggemann M, Rücklé C, Boerner S, Heidelberger JB, Busch A, Hänel H, Voigt A, Möckel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant J-Y, Zarnack K#, König J# and Beli P# (2019) The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation. Genome Biol, 20:216 (#indicates joint correspondence) Link
Borisova ME, Voigt A, Tollenaere MAX, Sahu SK, Juretschke T, Kreim N, Mailand N, Choudhary C, Bekker-Jensen S, Akutsu M, Wagner SA and Beli P (2018) p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage. Nat Commun, 9:1017 Link
Heidelberger JB, Voigt A, Borisova ME, Petrosino G, Ruf S, Wagner SA and Beli P (2018) Proteomic profiling of VCP substrates links VCP to K6‐linked ubiquitylation and c‐Myc function. EMBO Rep, 19:e44754 Link
Heidelberger JB, Wagner SA and Beli P (2016) Mass spectrometry-based proteomics for investigating DNA damage-associated protein ubiquitylation. Front Genet, 7:109 Link