Regulation of cell fate and gene silencing by liquid-liquid phase separation
The major focus of my lab is gene regulation by small RNAs. Small RNAs can have relatively minor effects on translation (in the case of miRNAs) to full-blown shutdown of loci at the transcriptional level (piRNAs). We use transgenics, genetic screens and biochemical and structural approaches to unravel the molecular pathways that are steered by small RNA guides in embryonic and germ cell development.
We are currently working to understand how piRNA responses can be initiated de novo and how and when a transient small RNA-mediated response can be transformed into a stably inherited response. In addition, we will start to work on understanding how small RNA pathways connect to other aspects of the cell's gene-regulatory programmes, including those active during germ cell specification and differentiation.
Positions held
2015 - 2017: Executive Director, Institute of Molecular Biology (IMB), Mainz
Since 2012: Scientific Director, IMB, Mainz
Professor, Faculty of Biology, Johannes Gutenberg University Mainz (JGU)
2010 - 2013: Professor of Epigenetics in Development, University of Utrecht
2005 - 2012: Group Leader, Hubrecht Institute, Utrecht
2000 - 2004: Postdoctoral Researcher, Hubrecht Laboratory, Utrecht
2000: Postdoctoral Researcher, Cold Spring Harbor Laboratories
Education
2000: PhD in Molecular Biology, Netherlands Cancer Institute, Amsterdam
1994: Masters in Chemistry, University of Leiden
Selected publications publications by René Ketting
Placentino M, de Jesus Domingues AM, Schreier J, Dietz S, Hellmann S, de Albuquerque BFM, Butter F and Ketting RF (2020) Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo. EMBO J, doi: 10.15252/embj.2020105280 Link
Redl S*, de Jesus Domingues AM*, Caspani E, Möckel S, Salvenmoser W, Mendez-Lago M and Ketting RF (2020) Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish. Development, doi: 10.1242/dev.193060 (*indicates joint contribution) Link
Almeida MV, de Jesus Domingues AM and Ketting RF (2019) Maternal and zygotic gene regulatory effects of endogenous RNAi pathways. PLOS Genet, 15:e1007784 Link
Almeida MV, Dietz S, Redl S, Karaulanov E, Hildebrandt A, Renz C, Ulrich HD, König J, Butter F and Ketting RF (2018) GTSF‐1 is required for formation of a functional RNA‐dependent RNA Polymerase complex in Caenorhabditis elegans. EMBO J, 37:e99325 Link
Lebedeva S, de Jesus Domingues AM, Butter F and Ketting RF (2017) Characterization of genetic loss-of-function of Fus in zebrafish. RNA Biol, 14:29–35 Link