High Resolution Fluorescence Imaging of Protein Plasticity

1 PhD project offered in the IPP winter call 2022

Scientific background

In higher eukaryotes, proteins that lack apparent folded structure are enriched in many vital processes. The Lemke group studies interactions between intrinsically disordered proteins and structured proteins in vital cellular processes, such as gene regulation, nuclear transport, host pathogen interactions and healthy aging. Since such highly dynamic systems are inaccessible by most conventional approaches, the group applies a multidisciplinary approach combining single molecule, microfluidics, super-resolution techniques and chemical/synthetic biology tools.

PhD project: High Resolution Fluorescence Imaging of Protein Plasticity

We are now looking for a PhD student with a strong interest in further expanding our efforts in quantitative single molecule and super resolution based technologies to study protein dynamics.

The project is particularly well suited for candidates with a strong background in the physical sciences, who are strongly interested in biosciences. However, the project will be highly interdisciplinary, and there are multiple aspects to it, that can be filled by one or two different persons with different interests/backgrounds.

In all cases, extensive project training will be provided that candidates with little or even prior no-hands on experience in the experimental biosciences but the strong wish to engage into those are particularly encouraged to apply. High interest and motivation are the main selection criteria and candidates from other disciplines that want to venture into these areas are explicitly welcome to apply.

Publications relevant for this project

Deciphering the conformations and dynamics of FG-nucleoporins in situ. M. Yu, M. Heidari, S. Mikhaleva, P.S. Tan, S. Mingu, H. Ruan, C.D. Reinkermeier, A. Obarska-Kosinska, M. Siggel, M. Beck, G. Hummer, E.A. Lemke. bioRxiv 2022.07.07.499201; doi: https://doi.org/10.1101/2022.07.07.499201

Christopher D. Reinkemeier, Edward A. Lemke (2021) Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation, Cell 184(19), p. 4886-4903.e21, Cell Press, doi:10.1016/J.CELL.2021.08.001

Ivana Nikić, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia V. Shymanska, Camilla Ventura Santos, Daniel Spitz, Edward A. Lemke (2016) Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy, Angewandte Chemie (International ed. in English) 55(52), p. 16172-16176, Angew Chem Int Ed Engl, doi:10.1002/ANIE.201608284


Prof. Dr. Edward A Lemke
Webpage: https://www.imb.de/lemke