CHEMICAL/CHEMICAL BIOLOGY/SYNTHETIC BIOLOGY TOOL DEVELOPMENT FOR HIGH RESOLUTION FLUORESCENCE STUDIES

2 PhD projects offered in the IPP winter call 2020/2021

Scientific Background

In higher eukaryotes, proteins that lack apparent folded structure are enriched in many vital processes. The Lemke group studies interactions between intrinsically disordered proteins and structured proteins in vital cellular processes, such as gene regulation, nuclear transport, and host pathogen interactions. Since such highly dynamic systems are inaccessible by most conventional approaches, the group applies a multidisciplinary approach combining single molecule, microfluidics, super-resolution techniques and chemical/synthetic biology tools.

PhD Projects

As part of an ERC funded project on “Multiple synthetic organelle design” we are now looking for PhD students with a strong interest in further expanding chemical/ chemical biology/ synthetic biology tools to develop next generation quantitative single molecule and super resolution based technologies.

The project is particularly well suited for candidates with a strong background in synthetic organic chemistry, chemical biology, synthetic biology, protein engineering, protein evolution, molecular biology and/or cell biology. However, the project will be highly interdisciplinary, and there are multiple aspects to it, that can be filled by one or two different persons with different interests/backgrounds.

In all cases, extensive project training will be provided that candidates with little or even prior no-hands on experience in the experimental biosciences but the strong wish to engage into those are particularly encouraged to apply. High interest and motivation are the main selection criteria and candidates from other disciplines that want to venture into these areas are explicitly welcome to apply.

Publications relevant to these projects

Reinkemeier CD, Estrada Girona G, Lemke EA (2019) Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes. Science, Mar 29;363(6434).

Nikić I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska N V., Ventura Santos C, Spitz D, Lemke EA (2016) Debugging eukaryotic genetic code expansion for site-specific click-PAINT super-resolution microscopy. Angew Chemie Int Ed, 55:16172-16176.

Nikić I, Plass T, Schraidt O, Szymański J, Briggs JAG, Schultz C, Lemke EA (2014) Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy. Angew Chemie Int Ed, 53:2245-2249.

 

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