Synthetic biophysics of protein disorder

Intrinsically disordered proteins (IDPs) are dynamic proteins that do not have a single well-defined structure, but instead adopt a range of different conformations. Because of this, IDPs are extremely hard to study. In my lab, we use cutting-edge chemical and synthetic biology, microfluidics, and super-resolved microscope engineering to develop innovative techniques to study IDPs in vital cellular processes like genome regulation. This includes engineering designer organelles to build synthetic proteins with fluorescent labels at specific sites, which enables us to better visualise their conformational changes at high resolution down to the single molecule level.

Research website

Positions held

Since 2018: Adjunct Director, Institute of Molecular Biology (IMB) & Professor of Synthetic Biophysics, Johannes Gutenberg University, (JGU), Mainz
2009 - 2020: Group Leader, EMBL, Heidelberg, Germany
2005-2008:  Research Associate (Postdoc), The Scripps Research Institute, La Jolla, CA

Education

2005: PhD MPI for Biophysical Chemistry and University of Göttingen, Germany
2001: Diploma in Chemistry, Technical University of Berlin, Germany
2001: Master of Science, University of Oklahoma, USA

Selected publications by Edward Lemke

Lu M, Ma X, Castillo-Menendez LR, Gorman J, Alsahafi N, Ermel U, Terry DS, Chambers M, Peng D, Zhang B, Zhou T, Reichard N, Wang K, Grover JR, Carman BP, Gardner MR, Nikić-Spiegel I, Sugawara A, Arthos J, Lemke EA, Smith AB, Farzan M, Abrams C, Munro JB, McDermott AB, Finzi A, Kwong PD, Blanchard SC, Sodroski JG and Mothes W (2019) Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRETNature, 568:415–419 Link

Chowdhury A, Kovalenko SA, Aramburu IV, Tan PS, Ernsting NP and Lemke EA (2019) Mechanism-dependent modulation of ultrafast interfacial water dynamics in intrinsically disordered protein complexesAngew Chemie Int Ed, 58:4720–4724 Link

Rienkemeier CD, Girona GE and Lemke EA (2019) Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotesScience, 363:eaaw2644 Link

Tan PS, Aramburu IV, Mercadante D, Tyagi S, Chowdhury A, Spitz D, Shammas SL, Gräter F and Lemke EA (2018) Two differential binding mechanisms of FG-nucleoporins and nuclear transport receptorsCell Rep, 22:3660–3671 Link

Koehler C, Sauter PF, Wawryszyn M, Girona GE, Gupta K, Landry JJM, Fritz MH-Y, Radic K, Hoffmann J-E, Chen ZA, Zou J, Tan PS, Galik B, Junttila S, Stolt-Bergner P, Pruneri G, Gyenesei A, Schultz C, Biskup MB, Besir H, Benes V, Rappsilber J, Jechlinger M, Korbel JO, Berger I, Braese S and Lemke EA (2016) Genetic code expansion for multiprotein complex engineeringNat Methods, 13:997–1000 Link



Lemke lab website
EMBL website