Impairment of transcription by DNA damage

DNA damage induced by environmental genotoxic agents unavoidably leads to stochastic mutations. Intriguingly, genes with mutations in human diseases (like cancers, neurological syndromes and immune disorders) often have abnormal epigenetic patterns as well, suggesting that both genetic and epigenetic alterations originate from common DNA damage events earlier in life.

My team investigates the effects of DNA damage and repair on the local epigenetic landscape and gene transcription in order to identify factors contributing to gene dysfunction in age-related diseases and hereditary diseases with progeroid phenotype (such as Cockayne syndrome).

Research website

Positions held

Since 2016: Group Leader (Heisenberg Fellowship), Genetic Toxicology, University Medical Center Mainz
2005-2016: Research Scientist, Genetic Toxicology, University of Mainz (habilitation in 2013)
2001-2005: Researcher, Molecular Biology, University of Bologna
2000-2001: IARC Research Fellow, Cancer Biology, University of Bologna
1999-2000: Junior Research Associate, R.E. Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, Kyiv

Education

1999: PhD in Biology (Oncology), R.E.Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, Kyiv
1995: Diploma (honours) in Biology / Immunology, National Taras Shevchenko University of Kyiv

 

Selected publications by Andriy Khobta

Kitsera N, Rodriguez-Alvarez M, Emmert S, Carell T, Khobta A (2019) Nucleotide excision repair of abasic DNA lesions. Nucleic Acids Research, 1-12 Link

Kitsera, N, Allgayer, J, Parsa, E, Geier, N, Rossa, M, Carell, T, Khobta A (2017) A Functional impacts of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine at a single hemi-modified CpG dinucleotide in a gene promoterNucleic Acids Res, 45(19):11033-11042 Link

Allgayer J, Kitsera N, Bartelt S, Epe B and Khobta A (2016) Widespread transcriptional gene inactivation initiated by a repair intermediate of 8-oxoguanine. Nucleic Acids Res, (44) 7267-7280 Link

Kitsera N, Gasteiger K, Lühnsdorf B, Allgayer J, Epe B, Carell T, Khobta A (2014) Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts from transcribed DNA. PLoS One, (9) e94405 Link

Lühnsdorf B, Epe B and Khobta A (2014) Excision of uracil from transcribed DNA negatively affects the gene expression. J Biol Chem, 289, 22008-22018 Link